Journal: BMC Cell Biology

Article Title: Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells

doi: 10.1186/1471-2121-7-30

Figure Lengend Snippet: Purity assessment of the CD34+ cell fraction by flow cytometry . The initial purity of CD34+ cells after separation through single column was 47.5%. The CD34- fraction was 99.4% pure. CD34+ and CD34- cell populations were defined by first gating on forward and side scatter properties excluding platelets and debris. Subsequent gates were set to exclude >99% of control cells labeled with isotype-specific antibody. Percentages indicating the purity of isolated cell fractions are shown for both plots. Abbreviations: SSC, side scatter; PE, phycoerythrin.

Article Snippet: When using the Direct CD34 Progenitor Cell Isolation Kit with single column separation and the labeling protocol recommended by the manufacturer (Miltenyi Biotec, Bergisch Gladbach, Germany), a purity of less than 50% was reached for the CD34+ cells (Figure ).

Techniques: Flow Cytometry, Control, Labeling, Isolation

Journal: BMC Cell Biology

Article Title: Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells

doi: 10.1186/1471-2121-7-30

Figure Lengend Snippet: Purity assessment of CD34+ cell fraction after one or two column separations . A) The CD34+ cell fraction was 78% pure after the first column separation. B) A 92% pure CD34+ cell faction was obtained by an additional labeling step in connection with a second column separation. CD34+ cell populations were defined by first gating on forward and side scatter properties excluding platelets and debris. Subsequent gates were set to exclude >99% of control cells labeled with isotype-specific antibody. Percentages indicating the purity of isolated cell fractions are shown for both plots. Abbreviations: SSC, side scatter; PE, phycoerythrin.

Article Snippet: When using the Direct CD34 Progenitor Cell Isolation Kit with single column separation and the labeling protocol recommended by the manufacturer (Miltenyi Biotec, Bergisch Gladbach, Germany), a purity of less than 50% was reached for the CD34+ cells (Figure ).

Techniques: Labeling, Control, Isolation

Journal: BMC Cell Biology

Article Title: Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells

doi: 10.1186/1471-2121-7-30

Figure Lengend Snippet: Purity assessment of CD34+/-, CD133+/- and Lin-/+ cell fractions . A) Purities for CD34+ and CD34- cell factions were 97.1% and 99.1%, respectively. B) Purities for CD133+ and CD133- fractions were 93.6% and 99.1%, respectively. C) Purities for Lin- and Lin+ cell factions were 97.0% and 99.5%, respectively. CD34+/-, CD133+/- cell populations were defined by first gating on forward and side scatter properties excluding platelets and debris. Subsequent gates were set to exclude >99% of control cells labeled with isotype-specific antibody. Percentages indicating the purity of isolated cell fractions are shown for both plots. Abbreviations: SSC, side scatter; IgG, immunoglobulin; PE, phycoerythrin.

Article Snippet: When using the Direct CD34 Progenitor Cell Isolation Kit with single column separation and the labeling protocol recommended by the manufacturer (Miltenyi Biotec, Bergisch Gladbach, Germany), a purity of less than 50% was reached for the CD34+ cells (Figure ).

Techniques: Control, Labeling, Isolation

Journal: BMC Cell Biology

Article Title: Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells

doi: 10.1186/1471-2121-7-30

Figure Lengend Snippet: A chart of the optimized protocols to isolate CD34+/-, CD133+/- and Lin-/+ cells from cord blood . Isolation of CD34/- and CD133+/- cells was performed using Direct CD34 Progenitor Cell Isolation Kit (#130-046-702, Miltenyi Biotec) and CD133 Cell Isolation Kit (#130-050-801, Miltenyi Biotec), respectively. Lin-/+ cells were isolated using StemSep Human Progenitor Enrichment Kit (#14056, StemCell Technologies). For all magnetic separations, MACS columns and separators (Miltenyi Biotech) were used. Abbreviations: MNC, mononuclear cells; Buffer, PBS pH 7.2 supplemented with 0.5% bovine serum albumin and 2 mM EDTA or 0.6% ACD/A.

Article Snippet: When using the Direct CD34 Progenitor Cell Isolation Kit with single column separation and the labeling protocol recommended by the manufacturer (Miltenyi Biotec, Bergisch Gladbach, Germany), a purity of less than 50% was reached for the CD34+ cells (Figure ).

Techniques: Isolation, Cell Isolation

Journal: BMC Cell Biology

Article Title: Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells

doi: 10.1186/1471-2121-7-30

Figure Lengend Snippet: Frequency of different types of CFU colonies within CD34+, CD133+, Lin- and MNC populations.

Article Snippet: When using the Direct CD34 Progenitor Cell Isolation Kit with single column separation and the labeling protocol recommended by the manufacturer (Miltenyi Biotec, Bergisch Gladbach, Germany), a purity of less than 50% was reached for the CD34+ cells (Figure ).

Techniques:

Journal: Antioxidants

Article Title: A Randomised, Double-Blind, Placebo-Controlled, Cross-Over Clinical Trial to Evaluate the Biological Effects and Safety of a Polyphenol Supplement on Healthy Ageing

doi: 10.3390/antiox13080995

Figure Lengend Snippet: Baseline concentrations of the blood biomarkers.

Article Snippet: The plasma CD38 levels were measured using the Abcam’s Human CD38 ELISA kit (Abcam Limited, Waltham, MA, USA).

Techniques:

Journal: Antioxidants

Article Title: A Randomised, Double-Blind, Placebo-Controlled, Cross-Over Clinical Trial to Evaluate the Biological Effects and Safety of a Polyphenol Supplement on Healthy Ageing

doi: 10.3390/antiox13080995

Figure Lengend Snippet: Concentrations at baseline, at one week, and differences for each outcome.

Article Snippet: The plasma CD38 levels were measured using the Abcam’s Human CD38 ELISA kit (Abcam Limited, Waltham, MA, USA).

Techniques:

Journal: Antioxidants

Article Title: A Randomised, Double-Blind, Placebo-Controlled, Cross-Over Clinical Trial to Evaluate the Biological Effects and Safety of a Polyphenol Supplement on Healthy Ageing

doi: 10.3390/antiox13080995

Figure Lengend Snippet: Differences for each outcome at one hour, two hours, and twenty-four hours compared to baseline.

Article Snippet: The plasma CD38 levels were measured using the Abcam’s Human CD38 ELISA kit (Abcam Limited, Waltham, MA, USA).

Techniques:

Journal: Antioxidants

Article Title: A Randomised, Double-Blind, Placebo-Controlled, Cross-Over Clinical Trial to Evaluate the Biological Effects and Safety of a Polyphenol Supplement on Healthy Ageing

doi: 10.3390/antiox13080995

Figure Lengend Snippet: Graphs showing the means and standard deviations for each biomarker concentration for each treatment’s outcomes (combining the two treatment periods, e.g., active or placebo) across all available time points. All target analytes were measured at T0 (baseline) and at the end of each 1-week study period (D7). In addition, NAD+ was measured 1 (T1), 2 (T2), and 24 (T24) hours after the intake of the first capsule in each period. CD38 was also measured 24 h (T24) after the first capsule intake.

Article Snippet: The plasma CD38 levels were measured using the Abcam’s Human CD38 ELISA kit (Abcam Limited, Waltham, MA, USA).

Techniques: Biomarker Assay, Concentration Assay

Journal: International Journal of Stem Cells

Article Title: Generation of Organotypic Multicellular Spheres by Magnetic Levitation: Model for the Study of Human Hematopoietic Stem Cells Microenvironment

doi: 10.15283/ijsc18061

Figure Lengend Snippet: MSC characteristics isolated from human BM. (A) MSC Morphology (3 passage): cells with an eccentric nucleus, loose chromatin and nucleoli; presence of granular basophilic cytoplasm (×100 Cythospin, hematoxylin and eosin stain, optical microscopy. Scale bar: 20 μ m), (B) MSC Morphology (3 passage): Fibroblastoid cells associated with MSC (×20, inverted microscopy. Scale bar: 5 μ m), (C, D) Immunophenotype (3 passage): MSCs do not express hematopoietic antigens (CD34 and CD45) and co-express CD73 and CD105, (E) Osteogenic differentiation (×20, red alizarin stain, inverted microscopy. Scale bar: 5 μ m), (F) Adipogenic differentiation (×40, sudan black stain, inverted microscopy. Scale bar: 5 μ m), (G) Chondrogenic differentiation (×20, safranin O stain, inverted microscopy. Scale bar: 5 μ m).

Article Snippet: Mononuclear cells were isolated by Ficoll density gradient centrifugation (Histopaque d=1.077 g/cm 3 , Sigma-Aldrich), and CD34+ cells were isolated using a previously described magnetic-activated cell-sorting (MACS) CD34 isolation kit (Miltenyi Biotec) ( ).

Techniques: Isolation, H&E Stain, Microscopy, Inverted Microscopy, Staining

Journal: International Journal of Stem Cells

Article Title: Generation of Organotypic Multicellular Spheres by Magnetic Levitation: Model for the Study of Human Hematopoietic Stem Cells Microenvironment

doi: 10.15283/ijsc18061

Figure Lengend Snippet: Percentage of antigens evaluated in mesenchymal cells isolated from bone marrow

Article Snippet: Mononuclear cells were isolated by Ficoll density gradient centrifugation (Histopaque d=1.077 g/cm 3 , Sigma-Aldrich), and CD34+ cells were isolated using a previously described magnetic-activated cell-sorting (MACS) CD34 isolation kit (Miltenyi Biotec) ( ).

Techniques: Isolation

Journal: International Journal of Stem Cells

Article Title: Generation of Organotypic Multicellular Spheres by Magnetic Levitation: Model for the Study of Human Hematopoietic Stem Cells Microenvironment

doi: 10.15283/ijsc18061

Figure Lengend Snippet: Umbilical cord blood (UCB) donors for HSC isolation

Article Snippet: Mononuclear cells were isolated by Ficoll density gradient centrifugation (Histopaque d=1.077 g/cm 3 , Sigma-Aldrich), and CD34+ cells were isolated using a previously described magnetic-activated cell-sorting (MACS) CD34 isolation kit (Miltenyi Biotec) ( ).

Techniques:

Journal: International Journal of Stem Cells

Article Title: Generation of Organotypic Multicellular Spheres by Magnetic Levitation: Model for the Study of Human Hematopoietic Stem Cells Microenvironment

doi: 10.15283/ijsc18061

Figure Lengend Snippet: UCB-HSC and Ec characteristics (cell line CC-2811. Lonza ® ). (A) HSC Morphology: cells with a predominant nucleus, nucleoli and basophil cytoplasm (×100 Cythospin, hematoxylin and eosin stain, optical microscopy. Scale bar: 20 μ m), (B) Non-adherent cells compatible with UCB-HSC (×20, inverted microscopy. Scale bar: 5 μ m), (C) HSC phenotype: CD34+, (D) Ec Morphology: cells with metacentric nucleus and abundant cytoplasm (×100 Cythospin, hematoxylin and eosin stain, optical microscopy. Scale bar: 20 μ m), (E) Adherent cells compatible with Ec (×20, inverted microscopy. Scale bar: 5 μ m), (F) Ec phenotype: CD34+, CD31+, CD105+.

Article Snippet: Mononuclear cells were isolated by Ficoll density gradient centrifugation (Histopaque d=1.077 g/cm 3 , Sigma-Aldrich), and CD34+ cells were isolated using a previously described magnetic-activated cell-sorting (MACS) CD34 isolation kit (Miltenyi Biotec) ( ).

Techniques: H&E Stain, Microscopy, Inverted Microscopy

Journal: International Journal of Stem Cells

Article Title: Generation of Organotypic Multicellular Spheres by Magnetic Levitation: Model for the Study of Human Hematopoietic Stem Cells Microenvironment

doi: 10.15283/ijsc18061

Figure Lengend Snippet: Multipotent capacity of HSC after cultivation in OMS. (A) Dot plot representative of the recovery percentages of CD34+ cells after the disintegration of the multicellular spheres. The ability of HSC to generate (B) CFU-GM (colony forming unit - granulocyte/monocyte progenitor. ×10, inverted microscope. Scale bar: 5 mm) (C) Immature granulocytic and monocytic cells: promyelocytes, myelocytes, promonocytes and bands cells (×100, hematoxylin & eosin. Scale bar: 20 μ m), (D) CFU-E (colony forming unit - erythroid progenitor. ×10, inverted microscope. Scale bar: 5 mm), (E) Immature erythroid cells: orthochromatic normoblasts are indicated (×100, hematoxylin & eosin. Scale bar: 20 μ m), (F) CFU-GEMM (colony forming unit – granulocyte, erythrocyte, monocyte, megakaryocyte. ×10, inverted microscope. Scale bar: 5 mm) and (G) Immature cells of myeloid lineage: myeloblasts, promyelocytes, normoblasts, promonocytes (×100, hematoxylin & eosin. Scale bar: 20 μ m) was evaluated after 10 d (H) and 15d (I) (n=1) (p>0.05).

Article Snippet: Mononuclear cells were isolated by Ficoll density gradient centrifugation (Histopaque d=1.077 g/cm 3 , Sigma-Aldrich), and CD34+ cells were isolated using a previously described magnetic-activated cell-sorting (MACS) CD34 isolation kit (Miltenyi Biotec) ( ).

Techniques: Inverted Microscopy

Journal: Clinical & Translational Immunology

Article Title: Targeting CD38 with monoclonal antibodies disrupts key survival pathways in paediatric Burkitt's lymphoma malignant B cells

doi: 10.1002/cti2.70011

Figure Lengend Snippet: Correlation analysis of CD38 and MYC gene expression in pBL patient samples. (a) Unsupervised PCA analysis shows the cluster pattern of the 29 paediatric lymphoma samples (GSE10172) based on significantly differentially expressed genes among these samples (1800 genes, one‐way ANOVA with the post hoc Tukey test P = 0.01); the colour key for disease subtypes in this panel is consistent with those used in the following panels of this figure. (b) PCA chart shows the distribution of the significantly differentially expressed genes that lead to the samples clustering in a . (c) Heatmap shows the 1800 significantly differentially expressed genes and the hierarchical distribution of the samples reflecting the clustering in a . The disease subtype and absence/presence of the IgH‐MYC translocation related to these samples are also reported. Correlation analysis for MYC and CD38 gene expression was performed on the samples contained in the dataset GSE10172 and Pearson's correlation coefficient ( r ), coefficient of determination ( R 2 ) and P ‐value were calculated for (d) the whole 29 samples contained in the data set, (e) only for BL and BL‐like samples, (h) only for BL samples. (g) Same analysis as in (d–f) but on 11 pBL samples contained in the GSE64905 dataset. (h) Same analysis as in (d–g) but on 19 pBL samples contained in GSE10172 and GSE64905 data sets. Here, the data sets were merged and normalised using the Z ‐score normalisation method.

Article Snippet: The CD38 cyclase assay was conducted using the CD38 Inhibitor Screening Assay Kit (BPS Bioscience, San Diego, California, USA) according to the manufacturer's protocol.

Techniques: Expressing, Translocation Assay

Journal: Clinical & Translational Immunology

Article Title: Targeting CD38 with monoclonal antibodies disrupts key survival pathways in paediatric Burkitt's lymphoma malignant B cells

doi: 10.1002/cti2.70011

Figure Lengend Snippet: DARA and ISA differential interaction with CD38's structure and its cyclase activity and impact on pBL cell proliferation, apoptosis and cell cycle. (a, b) The extracellular domain's structure of CD38 from two perspectives (top and 180° rotated bottom view, respectively), highlighting the epitopes recognised by DARA and ISA and the active site's location. (c) The crystal structure of the unbound CD38 ectodomain (PDB entry: 1YH3), showing the accessible active site. Arrows indicate the predicted binding sites for DARA and ISA. The dashed box indicates active site. A zoomed‐in view of the active site is provided in the adjacent box. (d) The crystal structure of CD38's ectodomain complexed with NAD + (upper panel) and ADPR (lower panel) in its active site (PDB entries: 3OFS and 8P8C respectively). The dashed box indicates active site, NAD + and ADPR. A zoomed‐in view of the active site is provided in the adjacent box. (e, f) The conformational changes of CD38 when bound to the Fab region of DARA (PDB entry: 7DHA) and ISA (PDB entry: 4CMH), respectively. Dashed boxes indicate the active site. A zoomed‐in view of the active site is provided in the adjacent boxes. (g) Concentration‐dependent inhibition of CD38 cyclase activity by DARA, ISA and quercetin, a CD38 cyclase inhibitor. The assay was performed on recombinant CD38 protein, with untreated controls (represented by the blue dotted line, No inh.) set as 100%. (h, i) The cell number and % of dead cells (7AAD + ) analysis in Ramos cells after a 4‐day culture period, comparing untreated (−) to treated with DARA, ISA or beriglobin control. (j) Cell cycle stages in Ramos cells stained with Vibrant DyeCycle in the same experimental setup as (h, i) . (k) % of cells in G1, S and G2/M phase. Statistical significance was calculated with one‐way ANOVA with the post hoc Tukey test and denoted as * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data are presented as mean ± SD. Non‐significance is not indicated in the figure. Data in (g) are from two independent experiments with n = 3 replicates each, with results normalised and combined. Data in (h–k) are representative of one experiment with n = 3 replicates. Independent experiments were repeated at least twice.

Article Snippet: The CD38 cyclase assay was conducted using the CD38 Inhibitor Screening Assay Kit (BPS Bioscience, San Diego, California, USA) according to the manufacturer's protocol.

Techniques: Activity Assay, Binding Assay, Concentration Assay, Inhibition, Recombinant, Control, Staining

Journal: Clinical & Translational Immunology

Article Title: Targeting CD38 with monoclonal antibodies disrupts key survival pathways in paediatric Burkitt's lymphoma malignant B cells

doi: 10.1002/cti2.70011

Figure Lengend Snippet: Comparative efficacy of DARA and ISA on modulating IgM:CD19 and IgD:CD19 interactions. (a) Fab‐PLA study of the proximity of IgM to CD19 on Ramos cells unstimulated or 5‐min anti‐IgM–stimulated without and with exposure to DARA or ISA (top to bottom). PLA signals are shown in red and nuclei in blue. Scale bar, 5 μm. (b) Scatter dot plot represents the mean of PLA signals for IgM:CD19 interaction (signal counts). (c) Fab‐PLA study of the proximity of IgD to CD19 on Ramos cells unstimulated or 5‐min anti‐IgM–stimulated without and with exposure to DARA or ISA (top to bottom). PLA signals are shown in red and nuclei in blue. Scale bar, 5 μm. (d) Scatter dot plot represents the mean of PLA signals for IgD:CD19 interaction (signal counts). Shown are representative microscope images (a, c) . Statistical significance in this figure was calculated with one‐way ANOVA with the post hoc Tukey test and denoted as * P < 0.05; ** P < 0.01; **** P < 0.0001. Non‐significance is not indicated in the figure. Independent experiments were repeated twice. In these graphs, every data point is one cell; error bars show mean ± SD. (e) Schematic representation of IgM and IgD interactions with CD19 and CD38 upon BCR activation, detailing the distinct inhibitory impacts of DARA and ISA.

Article Snippet: The CD38 cyclase assay was conducted using the CD38 Inhibitor Screening Assay Kit (BPS Bioscience, San Diego, California, USA) according to the manufacturer's protocol.

Techniques: Microscopy, Activation Assay

Journal: Clinical & Translational Immunology

Article Title: Targeting CD38 with monoclonal antibodies disrupts key survival pathways in paediatric Burkitt's lymphoma malignant B cells

doi: 10.1002/cti2.70011

Figure Lengend Snippet: Anti‐CD38 mAbs impair PI3K pathway signalling in pBL cells. (a, b) Time‐course analysis of SYK phosphorylation levels post anti‐CD38 mAb treatment over 24 h. (c) Comparative kinetic analysis of SYK dephosphorylation between DARA and ISA‐treated cells. (d, e) The phosphorylation status of AKT over a 24‐h period post anti‐CD38 mAb treatment. (f) Differential analysis of pAKT level kinetics between DARA and ISA treatments. Statistical significance was assessed using one‐way ANOVA with the post hoc Tukey test comparing untreated vs treated, indicated by: * P < 0.05; ** P < 0.01; *** P < 0.001. In (c) and (f) , the statistical significance was assessed using the unpaired t ‐test for the comparison between DARA vs ISA in each time point and the paired t ‐test for the comparison of the whole kinetic, and indicated by * P < 0.05; *** P < 0.001. Data are presented as mean ± SD. Non‐significance is not denoted. Data are representative of two independent experiments. All results were normalised and merged for consistent interpretation. (g) Schematic representation of the proposed effect of anti‐CD38 mAb (ISA) treatment on MYC/PI3K pathways and consequences on the proliferation/apoptosis balance.

Article Snippet: The CD38 cyclase assay was conducted using the CD38 Inhibitor Screening Assay Kit (BPS Bioscience, San Diego, California, USA) according to the manufacturer's protocol.

Techniques: De-Phosphorylation Assay, Comparison

Journal: The Journal of pathology

Article Title: APRIL/BAFF upregulation is associated with clonal B-cell expansion in Hunner-type interstitial cystitis.

doi: 10.1002/path.6353

Figure Lengend Snippet: Figure 1. Immune profiling of HIC-L, HIC-NL, and non-HIC samples. (A) Schematic illustration of HIC-L and HIC-NL biopsies. (B) Box plots showing the proportions of T lymphocytes, B lymphocytes, and plasma cells calculated by CIBERSORTx in HIC-L specimens (n = 37), patient- matched HIC-NL specimens (n = 37), and non-HIC specimens (n = 24). Asterisks indicate a significant difference (p < 0.05) (C) Hematoxylin and eosin staining of an FFPE section from a representative patient with HIC. (D) Images reconstructed from mass cytometry analysis of the FFPE section from the same patient. Each color indicates a different immune cell type. Blue represents unannotated cells. (E) Bar charts showing the proportion of isotypes (heavy chains) in each sample. Colored boxes in the upper panel indicate dominant isotypes. Within each dominant isotype, samples are arranged from the highest to the lowest proportion of the dominant isotype. BCG, Chr cys, and Follicular refer to BCG cystitis, chronic cystitis, and follicular cystitis, respectively. (F) Bar charts showing the proportion of isotypes (light chains) in each sample. Within each condition, samples are arranged from the highest to the lowest proportion of IgK. Dark brown and light brown boxes in the upper panel indicate IgK light chain restriction (IgK/IgL > 5.5) and IgL light chain restriction (IgK/IgL < 0.7), respectively. BCG, Bacillus Calmette–Guérin; N.S., not significant; FFPE, formalin-fixed, paraffin-embedded; HIC, Hunner-type interstitial cystitis; HIC-L, Hunner lesion of Hunner-type interstitial cystitis; HIC-NL, non-Hunner lesion of Hunner-type interstitial cystitis.

Article Snippet: In brief, the FFPE section was stained using a Human Maxpar Immuno-Oncology Imaging Mass Cytometry Panel Kit (#201508; Fluidigm, South San Francisco, CA, USA) and 141Pr-CD38 (#3141018D; Fluidigm), following the manufacturer’s protocol.

Techniques: Clinical Proteomics, Staining, Mass Cytometry

Journal: Antioxidants

Article Title: Oxidative Stress and Inflammatory Markers in Abdominal Aortic Aneurysm

doi: 10.3390/antiox10040602

Figure Lengend Snippet: Circulating levels of IgM and IgG are altered in AAA patients. ( A , B ) Circulating levels of IgM and IgG, respectively in AAA ( n = 94) vs. healthy donors ( n = 46). ( C ) Representative images of immunostaining assays performed in abdominal aorta sections from donors and AAA patients targeting IgGs ( n = 10; scale bars: 100 µm). AAA (-) indicates negative control for immunohistochemistry. Arrows indicate the positively stained cells.

Article Snippet: The circulating levels of soluble IgM (ab214568, Abcam, UK), IgG (ab195215), soluble CD38 (KIT10818-1, Sino Biological Inc., Wayne, PA, USA), soluble CD36 (ABE-196-02, Nordic BioSite, Täby, Sweden), S100A4 (CSB-EL020632HU, Cusabio Biotech Co, LTD, Beijing, China) and GDF15 (Quantikine ELISA Human GDF15, DGD150; R&D Systems, Minneapolis, MN, USA), in plasma from patients were measured using commercially available ELISA kits in accordance with the manufacturer’s instructions.

Techniques: Immunostaining, Negative Control, Immunohistochemistry, Staining

Journal: Antioxidants

Article Title: Oxidative Stress and Inflammatory Markers in Abdominal Aortic Aneurysm

doi: 10.3390/antiox10040602

Figure Lengend Snippet: IgG, CD38 and GDF15 circulating levels positively correlate with the AAA diameter whereas only CD38 correlates with PWS. ( A ) Graphs showing the correlation analysis between IgG plasma levels and AAA diameter ( n = 90) and ( B , C ) the correlation analysis between CD38 plasma levels and AAA diameter ( n = 90) or PWS values in AAA patients ( n = 58). ( D ) Graph showing the correlation analysis between GDF15 plasma levels and AAA diameter ( n = 90). The r and p -values are obtained by performing the Spearman or the Pearson correlation coefficient test. Results are expressed as mean ± SEM.

Article Snippet: The circulating levels of soluble IgM (ab214568, Abcam, UK), IgG (ab195215), soluble CD38 (KIT10818-1, Sino Biological Inc., Wayne, PA, USA), soluble CD36 (ABE-196-02, Nordic BioSite, Täby, Sweden), S100A4 (CSB-EL020632HU, Cusabio Biotech Co, LTD, Beijing, China) and GDF15 (Quantikine ELISA Human GDF15, DGD150; R&D Systems, Minneapolis, MN, USA), in plasma from patients were measured using commercially available ELISA kits in accordance with the manufacturer’s instructions.

Techniques: